1. SnapGene Viewer. SnapGene Viewer lets you see your data for free with the same rich visualization, annotation, and sharing capabilities as the fully enabled SnapGene software. SnapGene is so easy to use that my lab adopted it instantly. It saves a lot of time and money as we now do all our cloning in SnapGene first and catch any snags in the.
  2. Gene-specific primers were designed using Gene Runner software and the mpt51 gene was amplified by PCR. The amplified fragment and pcDNA3.1(+) cloning vector were both digested with restriction enzymes, the mpt51 fragment was ligated into the vector, and the Escherichia coli (E. Coli) TOP10 strain were transformed by the recombinant plasmid.
  3. Amplification of chitinase Gene of. Beauveria bassiana. By Polymerase chain reaction: For the amplification of desired gene Bbchit1, the specific primer (Table 1) were designed by Gene runner software and used to amplify the sequence of open reading frame (ORF) of gene. A reaction mixture of PCR was prepared by adding the desired chemicals in.
  • Gene runner for windows 7

The primers were designed for all five exons and intron-exon boundaries of the TYR gene (NG008748.1) using gene runner software and also the primer blast website to prevent amplifying the TYRL pseudogene, which is similar to the 3′ half of this gene (Table1). These primers were used for PCR amplification and cycle sequencing.

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